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KMID : 0360220150560121939
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Journal of the Korean Ophthalmological Society
2015 Volume.56 No. 12 p.1939 ~ p.1944
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Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts
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황석진:Hwang Seok-Jin
김근해:Kim Keun-Hae
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Abstract
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Purpose: To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts.
Methods: Primary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 μg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining.
Results: AME decreased significantly in fibroblast proliferation after exposure to 10 μg/mL (p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 μg/mL (p = 0.024).
Conclusions: AME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes.
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KEYWORD
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Amniotic membrane extract, Apoptosis, Nasal mucosa fibroblast
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